Composite

Part:BBa_K1461303

Designed by: Shunsuke Sumi   Group: iGEM14_UT-Tokyo   (2014-09-26)


RBS-GFP-4 miR 142 5p non-binding site-d.term

This is used as control for characterization of translation inhibition by miR-142-5p in HL60. This contains RBS, GFP, 4 miR-142-5p non-binding sites and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


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Parameters
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